Deleted
Sample Discontinued
Experience: None
No Sequence
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Regulatory

Part:BBa_J22102:Design

Designed by: Krithiga Sankaran   Group: iGEM2006_Bangalore   (2006-09-04)


rec A (SOS) Promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

this is the description of the primers used for PCR amplification Forward Primer

GAATTCGCGGCCGCTTCTAG AACAATTTCTACAAAACACTTGATACTGT

Tm: 59.43 Length: 29 GC%:27.59

Reverse Primer

CTGCAGGCGGCCGAACTAGTT GCATGATGGAGCCTTTACC

Tm: 55.2 Length: 19 GC%: 56.63

Source

The Rec A(SOS) promoter from the chromosomal DNA from MG1655(E coli) has been PCR amplified and inserted into plasmid pSB1A2 (part number BBa_B0015).

References